Formal estimation of the seropositivity cut‐off of the hemagglutination inhibition assay in field diagnosis of influenza D virus in cattle and estimation of the associated true prevalence in Morocco
| Autor: | Zaid Zouagui, Hicham Ait Lbacha, Said Alali, Gilles Meyer, Elias Salem, Claude Saegerman, Mariette F. Ducatez |
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| Přispěvatelé: | Fundamental and Applied Research for Animals & Health (FARAH), Faculté de Médecine Vétérinaire [Liège], Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Agronomique et Vétérinaire Hassan II - IAV (MOROCCO) (IAV), This study was performed under the Grant Agreement Number GP/EFSA/AFSCO/2017/01-GA04, entitled ‘Risk assessment for influenza D in Europe'. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
040301 veterinary sciences [SDV]Life Sciences [q-bio] Cattle Diseases Bovine respiratory disease specificity virus Biology Spearman's rank correlation coefficient Virus 0403 veterinary science 03 medical and health sciences Orthomyxoviridae Infections Antigen Seroepidemiologic Studies Prevalence medicine Animals 030304 developmental biology 2. Zero hunger 0303 health sciences Hemagglutination assay General Veterinary General Immunology and Microbiology Receiver operating characteristic hemagglutination inhibition assay RNA virus 04 agricultural and veterinary sciences General Medicine Hemagglutination Inhibition Tests medicine.disease biology.organism_classification respiratory sensitivity 3. Good health Morocco Titer cattle surveillance epidemiology influenza Thogotovirus |
| Zdroj: | Transboundary and emerging diseases Transboundary and emerging diseases, Wiley-Blackwell, 2021, 68 (1), pp.1392-1399. ⟨10.1111/tbed.13805⟩ |
| ISSN: | 1865-1674 1865-1682 |
| Popis: | International audience; The influenza D virus (IDV) was discovered less than ten years ago. Increased interest in this virus is due to its nature (RNA virus with high mutation rate), its worldwide circulation in livestock species, its probable role in bovine respiratory disease and its zoonotic potential. Until currently, the establishment of positivity cut-off of the hemagglutination inhibition (HI) assay was not formalized in field conditions for the detection of antibodies directed against IDV in cattle (i.e. the proposed reservoir). In this study, the positivity cut-off of the HI assays was formally established (titre = 10) using a receiver operating characteristic (ROC) curve. This information was used to estimate the sensitivity (68.04 to 73.20%) and the specificity (94.17 to 96.12%) of two different HI assays (HI(1)and HI2, with two different IDV antigens) relatively to virus micro-neutralization test (VNT) as reference test. Based on the above characteristics, the true prevalence of IDV was then estimated in Morocco using a stochastic approach. Irrespective of the HI assays used, the estimation of the true prevalence was statistically equivalent (between 48.44% and 48.73%). In addition, the Spearman rank correlation between HI titres and VNT titres was statistically good (0.76 and 0.81 for HA(1)and HA(2), respectively). The positive (0.82 and 0.79 for HA(1)and HA(2), respectively) and the negative (0.86 and 0.85 for HA(1)and HA(2), respectively) agreement indices between results of HI assays and VNT were good and similar. This study allowed for a formal establishment of a positivity cut-off in HI assays for the detection of antibodies directed against IDV. This information is of prime importance to estimate the diagnostic sensitivity and specificity of the test relatively to the VNT (i.e. the reference test). Using these characteristics, the true prevalence of IDV should be determined in a country. |
| Databáze: | OpenAIRE |
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