Partial resolution of the sugar content of Toxoplasma gondii membrane
| Autor: | Michèle Dodeur, Roland Bourrillon, Geneviève Mauras, Jean-Marcel Senet, Patrice Laget |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Glycoconjugate
Biophysics Carbohydrates Biochemistry Cell membrane chemistry.chemical_compound Lectins parasitic diseases medicine Animals Molecular Biology Glycoproteins chemistry.chemical_classification biology Cell Membrane Toxoplasma gondii Membrane Proteins Cell Biology biology.organism_classification Wheat germ agglutinin Sialic acid medicine.anatomical_structure chemistry Concanavalin A Galactose oxidase biology.protein Sialic Acids Neuraminidase Toxoplasma |
| Zdroj: | Biochemical and biophysical research communications. 97(3) |
| ISSN: | 0006-291X |
| Popis: | The glycoconjugates on Toxoplasma gondii membrane have been investigated in several ways, usually used for the study of cell surfaces: binding of |14C| lectins, Concanavalin A (Con A) and Wheat germ agglutinin (WGA) to parasites; parasites membrane labelling, using galactose oxidase/tritiated sodium borohydride (NaB|3H4|); determination of sialic acid amount, sensitive to the neuraminidase. Toxoplasma gondii binds Con A but did not bind significantly WGA. The affinity constant for Con A binding was on the same order of magnitude as for other cell systems, but the number of sites per parasite was low (3×104 sites per parasite). In contrast, with other cell systems, no surface incorporation of tritium was obtained using galactose oxidase/NaB|3H4|. Moreover the determination of sialic acid, sensitive to the neuraminidase gave a negative result. These results shown that Toxoplasma gondii surface do not contain all sugars, usually found in oligosaccharidic chains of membrane glycoconjugates and suggest the presence of low amount of surface glycoconjugates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|