Reconstitution of purified cardiac muscle calcium release channel (ryanodine receptor) in planar bilayers
| Autor: | Lin Hymel, Sidney Fleischer, Makoto Inui, Hansgeorg Schindler |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Ruthenium red
Lipid Bilayers Biophysics chemistry.chemical_element Calcium Biochemistry Ryanodine receptor 2 chemistry.chemical_compound Adenosine Triphosphate Dogs medicine Animals Magnesium Receptors Cholinergic Lipid bilayer Molecular Biology Phospholipids Chemistry Ryanodine receptor Endoplasmic reticulum Myocardium Cardiac muscle Skeletal muscle Ryanodine Receptor Calcium Release Channel Cell Biology Ruthenium Red Molecular Weight Kinetics medicine.anatomical_structure Soybeans |
| Zdroj: | Biochemical and biophysical research communications. 152(1) |
| ISSN: | 0006-291X |
| Popis: | The purified ryanodine receptor of heart sarcoplasmic reticulum (SR) has been reconstituted into planar phospholipid bilayers and found to form Ca2+-specific channels. The channels are strongly activated by Ca2+ (10 nM) in the presence of ATP (1 mM) and ryanodine, and inactivated by Mg2+ (3 mM) or ruthenium red (30 microM). These characteristics are diagnostic of calcium release from heart SR. The cardiac ryanodine receptor, which has previously been identified as the foot structure, is now identified as the calcium release channel. A similar identity of the calcium release channel has recently been reported for skeletal muscle. The characteristics of the calcium release channel from skeletal muscle and heart are similar in that they: 1) consist of an oligomer of a single high molecular weight polypeptide (Mr 360,000 for skeletal muscle and 340,000 for heart); 2) exist morphologically as the foot structure; 3) are activated (ATP, Ca2+, ryanodine) and inhibited (ruthenium red and Mg2+) by a number of the same ligands. Important differences include: 1) Ca2+ activation at lower concentration of Ca2+ for the heart; 2) more dramatic stabilization by ryanodine of the open state for the skeletal muscle channel; and 3) different relative permeabilities (PCa/PK). |
| Databáze: | OpenAIRE |
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