Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine
| Autor: | Adolfo Ludovico Martino, Favio E. Pollo, Zulma Anahí Salinas, Pablo Raúl Grenat, Nancy Edith Salas, Manuel Alejandro Otero |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male LEUKOCYTE Otras Ciencias Biológicas Health Toxicology and Mutagenesis Argentina Mineralogy 010501 environmental sciences medicine.disease_cause 01 natural sciences Fluorite Mining Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound Fluorides medicine Environmental Chemistry Ecotoxicology Animals 0105 earth and related environmental sciences MICRONUCLEI biology Mutagenicity Tests General Medicine biology.organism_classification Pollution 030104 developmental biology Wastewater Rhinella arenarum chemistry Environmental chemistry Micronucleus test Bufo arenarum FLUORITE MINE NUCLEAR ABNORMALITIES Female Micronucleus Fluoride RHINELLA ARENARUM CIENCIAS NATURALES Y EXACTAS Genotoxicity Water Pollutants Chemical DNA Damage |
| Zdroj: | Environmental science and pollution research international. 24(22) |
| ISSN: | 1614-7499 |
| Popis: | Little attention has been paid to the impact of wastewater generated by mining activities on fluoride. In this study, we evaluated the hematology responses of common South American toad Rhinella arenarum inhabiting natural and artificial environments associated with a fluorite mine from central Argentina. We analyzed three sampling stations associated with the fluorite mine: (I) Los Cerros Negros stream (CN), which runs on granitic rock with a high fluorite content; (II) Los Vallecitos stream (LV), which runs on metamorphic rock with low fluorite content; and (III) artificial decantation ponds (DP) containing sediments produced by fluorite flotation process. We calculated frequencies of micronuclei, erythrocyte nuclear abnormalities, mitosis, and immature erythrocytes. In addition, we performed a differential leukocyte count and determined neutrophils/lymphocyte ratio as a stress response estimator. We found high micronucleus (MN) and erythrocyte nuclear abnormality (ENA) frequencies in DP and CN but low frequencies in LV. The neutrophil/lymphocyte ratio was different among sites, with a significant increase in individuals from DP. Values registered in DP could be caused by exposure to mixture of compounds registered in dams that hold wastewater, while high values registered in CN stream might be due to natural concentrations of fluoride. Our results suggest that blood is an effective and non-destructive sensitive indicator for monitoring genotoxic agents in freshwater ecosystems. Fil: Pollo, Favio Ezequiel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Grenat, Pablo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina Fil: Salinas, Zulma Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina Fil: Otero, Manuel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina Fil: Salas, Nancy Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina Fil: Martino, Adolfo Ludovico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina |
| Databáze: | OpenAIRE |
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