Mapping of the C1q Binding Site on Rituxan, a Chimeric Antibody with a Human IgG1 Fc
| Autor: | Michael G. Mulkerrin, L.G. Presta, Klara Totpal, Y.G. Meng, Esohe Ekinaduese Idusogie, H. Gazzano-Santoro, Pin Yee Wong, Mark Ultsch |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.drug_class
Phenylalanine Recombinant Fusion Proteins Guinea Pigs Immunology Antibody Affinity Monoclonal antibody Peptide Mapping Antibodies Monoclonal Murine-Derived Mice immune system diseases hemic and lymphatic diseases medicine Animals Humans Immunology and Allergy Binding site Receptor B cell CD20 Aspartic Acid Alanine biology Complement C1q Lysine Antibody-Dependent Cell Cytotoxicity Antibodies Monoclonal Molecular biology Immunoglobulin Fc Fragments Complement system medicine.anatomical_structure Amino Acid Substitution Immunoglobulin G biology.protein Rituximab Binding Sites Antibody Rabbits Antibody medicine.drug |
| Zdroj: | The Journal of Immunology. 164:4178-4184 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.164.8.4178 |
| Popis: | Rituxan (Rituximab) is a chimeric mAb with human IgG1 constant domains used in the therapy of non-Hodgkin’s B cell lymphomas. This Ab targets B cells by binding to the cell-surface receptor, CD20. In our investigation of the mechanism of B cell depletion mediated by Rituximab, we first constructed mutants of Rituximab defective in complement activation but with all other effector functions intact. Our results demonstrate that the previously described C1q binding motif in murine IgG2b constituting residues E318, K320, and K322 is not applicable to a human IgG1 when challenged with either human, rabbit, or guinea pig complement. Alanine substitution at positions E318 and K320 in Rituximab had little or no effect on C1q binding and complement activation, whereas alanine substitution at positions D270, K322, P329, and P331 significantly reduced the ability of Rituximab to bind C1q and activate complement. We have also observed that concentrations of complement approaching physiological levels are able to rescue >60% of the activity of these mutant Abs with low affinity for C1q. These data localize the C1q binding epicenter on human IgG1 and suggest that there are species-specific differences in the C1q binding site of Igs. |
| Databáze: | OpenAIRE |
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