The Saliva Cortisol and Amylase Levels Related With Stress Response Compared by Different Analytical Methods
| Autor: | Burak Arslan, Umut Kökbaş, Erdal Ergünol, Levent Kayrin, Aylin Sepici Dinçel, Rabia Şemsi |
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| Přispěvatelé: | Nevşehir Hacı Bektaş Veli Üniversitesi/diş hekimliği fakültesi/temel bilimler bölümü/temel tıp bilimleri anabilim dalı |
| Rok vydání: | 2021 |
| Předmět: |
Saliva
medicine.medical_specialty Chemiluminescence Hydrocortisone Amylase levels Bioengineering Stress Applied Microbiology and Biotechnology Biochemistry Cortisol law.invention Fight-or-flight response law Internal medicine medicine Amylase Elisa method Molecular Biology Cortisol level Salivary cortisol biology Chemistry General Medicine Endocrinology biology.protein ELISA Biosensor Biotechnology |
| Popis: | Sampling of salivary cortisol and amylase is a non-invasive method and important for the evaluation of the hypothalamic-pituitary-adrenal axis function and stress levels. This study aimed to compare the values of the salivary cortisol and amylase levels which were measured by three different analytical methods to discuss the alterations of stress levels of samples. The saliva samples of young adults (n = 23) were collected between 08.00 and 09.00 a.m., noon at 12.00 (before exam) and between 14.00 and 15.00 p.m. (after unaware exam). The samples were measured within the first 48 h, and no freezing/thawing was done. Salivary cortisol and amylase levels of subjects were measured by three different analytical methods as ELISA, chemiluminescence and biosensor methods. Comparison of ELISA and biosensor methods in order to determine the salivary cortisol levels showed a good correlation y = 2.971 + 0.748x (R-2 = 0.839). Salivary amylase concentrations were only detected by ELISA method. Biosensor can be offered as an alternative analytic method to the conventional determination method ELISA. It can be preferred because of the detection/information effectiveness, low cost, fast results and specificity characteristics. |
| Databáze: | OpenAIRE |
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