The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
| Autor: | Pablo E. Visconti, Maria Gracia Gervasi, Xinran Xu, Mariano G. Buffone, Diego Krapf, Blanca I. Carbajal-González |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Axoneme Male Protein Conformation alpha-Helical macromolecular substances Flagellum Biology ACTIN purl.org/becyt/ford/1 [https] Ciencias Biológicas 03 medical and health sciences Mice 0302 clinical medicine MITOCHONDRIA FERTILIZATION Animals Spectrin Cytoskeleton purl.org/becyt/ford/1.6 [https] Sperm motility 030304 developmental biology 0303 health sciences Sperm flagellum urogenital system Cell Biology Actin cytoskeleton Sperm Actins Cell biology Actin Cytoskeleton 030104 developmental biology Sperm Tail Biología Reproductiva 030217 neurology & neurosurgery CIENCIAS NATURALES Y EXACTAS SPERM Research Article |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1477-9137 |
| Popis: | Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility is one of the features of sperm that allows them to succeed in fertilization, and their flagellum is essential for this function. Longitudinally, the flagellum can be divided into the midpiece, the principal piece and the end piece. A precise cytoskeletal architecture of the sperm tail is key for the acquisition of fertilization competence. It has been proposed that the actin cytoskeleton plays essential roles in the regulation of sperm motility; however, the actin organization in sperm remains elusive. In the present work, we show that there are different types of actin structures in the sperm tail by using three-dimensional stochastic optical reconstruction microscopy (STORM). In the principal piece, actin is radially distributed between the axoneme and the plasma membrane. The actin-associated proteins spectrin and adducin are also found in these structures. Strikingly, polymerized actin in the midpiece forms a double-helix that accompanies mitochondria. Our findings illustrate a novel specialized structure of actin filaments in a mammalian cell. Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados Unidos Fil: Xu, Xinran. State University of Colorado - Fort Collins; Estados Unidos Fil: Carbajal Gonzalez, Blanca. Mount Holyoke College; Estados Unidos Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos Fil: Krapf, Diego. State University of Colorado - Fort Collins; Estados Unidos |
| Databáze: | OpenAIRE |
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