Induction of oxidative stress and apoptosis by pentachlorophenol in primary cultures of Carassius carassius hepatocytes
| Autor: | Xiaohu Zhao, Yu-Liang Dong, Peijiang Zhou, Xue-Wu Pan, Shun-Yao Jiang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Carps
Pentachlorophenol Time Factors Cell Survival Physiology Health Toxicology and Mutagenesis Carassius carassius Cell Culture Techniques Apoptosis Mitochondria Liver DNA Fragmentation DNA laddering Biology Toxicology medicine.disease_cause Biochemistry Lipid peroxidation chemistry.chemical_compound Adenosine Triphosphate Malondialdehyde medicine Animals Cells Cultured Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug Caspase 3 Cell Biology General Medicine Glutathione Molecular biology Oxidative Stress chemistry Hepatocytes Calcium Ca(2+) Mg(2+)-ATPase Lipid Peroxidation Reactive Oxygen Species Water Pollutants Chemical Intracellular Oxidative stress |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 150:179-185 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2009.04.010 |
| Popis: | Pentachlorophenol (PCP) is a highly toxic contaminant of chlorophenols. Due to its slow and incomplete biodegradation, it can be found in surface, groundwater and in soils. To investigate the role of intracellular calcium and reactive oxygen species in apoptosis induced by PCP in cultured hepatocytes, the primary hepatocytes of Carassius carassius were incubated with different concentrations of PCP at 25 degrees C for 8 h in vitro. Apoptosis was detected by DNA laddering, caspase activation and flow cytometry. The results demonstrated that apoptosis was involved in the cytotoxic effect of PCP, and that PCP-induced apoptosis occurred in a dose-dependent manner. In addition, the induction of apoptosis by PCP was accompanied with Ca2+, Mg2+-ATPase activity decline, intracellular Ca2+ elevation, generation of intracellular reactive oxygen species (ROS), mitochondrial membrane potential (DeltaPsi(m)) disruption and ATP depletion. Concomitantly, there were dose-dependent increases in lipid peroxidation production (MDA) and decreases in glutathione (GSH). These investigations suggest that PCP-induces apoptosis in the cultured hepatocytes by affecting multiple targets, and suggest that [Ca2+]i increase and ROS generation may be involved in apoptosis induction by PCP. |
| Databáze: | OpenAIRE |
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