Proteochondroitin sulfate synthesis and chondrogenic expression
| Autor: | John P. Pennypacker, Patricia D. Royal, Paul F. Goetinck |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Chick Embryo Biology Sulfur Radioisotopes Mesoderm Limb bud chemistry.chemical_compound Mucoproteins medicine Animals Cells Cultured Sulfates Cartilage Cell Differentiation Cell Biology Chondrogenesis Embryonic stem cell Cell biology medicine.anatomical_structure chemistry Biochemistry Chromatography Gel Agarose Chondroitin Proteochondroitin Sulfate |
| Zdroj: | Experimental Cell Research. 87:241-248 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(74)90476-5 |
| Popis: | Embryonic limb bud cells were cultured under conditions in which chondrogenic expression takes place. Quantitative and qualitative estimates were made of the proteochondroitin sulfate synthesized under these conditions. A tenfold increase in incorporation into proteochondroitin sulfate is observed over the culture period during which chondrogenic expression takes place. This increase is reflected in a preferential increase of a particular fraction identified on 1% agarose chomatography. Low levels of this chromatographic fraction can be identified in limb buds before any evidence of cartilage expression is evident. We postulate that this preferential augmentation is a cartilage specific event. |
| Databáze: | OpenAIRE |
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