Characterization of recombinant pumpkin 2S albumin and mutation studies to unravel potential DNA/RNA binding site
| Autor: | Brajesh Kumar Savita, Shweta Choudhary, Partha Pratim Roy, Ashwani Sharma, Neeladrisingha Das, Pravindra Kumar, Vikram L. Dalal, Deena Nath Gupta, Shailly Tomar |
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| Rok vydání: | 2021 |
| Předmět: |
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Molecular RNase P Biophysics medicine.disease_cause Biochemistry law.invention chemistry.chemical_compound Anti-Infective Agents Cucurbita law Albumins medicine Binding site Molecular Biology Plant Proteins Mutation Nuclease biology Mutagenesis RNA Cell Biology DNA Recombinant Proteins chemistry Seeds Recombinant DNA biology.protein Protein Binding |
| Zdroj: | Biochemical and biophysical research communications. 580 |
| ISSN: | 1090-2104 |
| Popis: | The native pumpkin 2S albumin, a multifunctional protein, possess a variety of potential biotechnologically exploitable properties. The present study reports the characterization of recombinant pumpkin 2S albumin (rP2SA) and unraveling of its potential DNA/RNA binding site. The purification and characterization of the rP2SA established that it retains the characteristic α-helical structure and exhibited comparable DNase, RNase, antifungal and anti-proliferative activities as native protein. In vitro studies revealed that rP2SA exhibits potent antiviral activity against chikungunya virus (CHIKV) at a non-toxic concentration with an IC50 of 114.5 μg/mL. In silico studies and site-directed mutagenesis were employed to unravel the potential DNA/RNA binding site. A strong positive charge distribution due to presence of many arginine residues in proximity of helix 5 was identified as a potential site. The two of the arginine residues, conserved in some 2S albumins, were selected for the mutation studies. The mutated forms of recombinant protein (R84A and R91A) showed a drastic reduction in DNase and RNase activities suggesting their presence at binding site and involvement in the nuclease activity. A metal binding site was also identified adjacent to DNA/RNA binding site. The present study demonstrated the structural and functional integrity of the rP2SA and reports potential antiviral activity against CHIKV. Further, potential DNA/RNA binding site was unraveled through mutation studies and bioinformatics analysis. |
| Databáze: | OpenAIRE |
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