Immune regulation by CD4+CD25+T cells and interleukin-10 in birch pollen-allergic patients and non-allergic controls
| Autor: | C. Trollmo, M. Akdis, Cezmi A. Akdis, R. Grönneberg, Guro Gafvelin, Sarah Thunberg, M. van Hage, V. Malmstrom |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Allergy medicine.medical_treatment Immunology Biology T-Lymphocytes Regulatory Th2 Cells Immune system Antigen Hypersensitivity Immune Tolerance medicine Homeostasis Humans Immunology and Allergy IL-2 receptor Betula Cells Cultured T lymphocyte Allergens Middle Aged Th1 Cells medicine.disease Coculture Techniques Interleukin-10 Interleukin 10 Cytokine Cell culture Cytokines Pollen Female |
| Zdroj: | Clinical & Experimental Allergy. 37:1127-1136 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2007.02739.x |
| Popis: | Background CD4 + CD25 + regulatory T (Treg) cells and the cytokines IL-10 or TGF-β play key roles in the maintenance ofT cell homeostasis and tolerance to infectious and non-infectious antigens such as allergens. Objective To investigate the regulation of immune responses to birch pollen allergen compared with influenza antigen by Treg cells obtained from birch pollen-allergic patients and non-allergic controls. Methods Peripheral blood was collected from 10 birch pollen-allergic patients and 10 non-allergic healthy controls. CD4 + CD25 + and CD4 + CD25 - cells isolated by magnetic-activated cell sorting were co-cultured and stimulated with birch pollen extract or influenza vaccine in the absence or presence of anti-IL-10 or soluble TGF-βRII. Results CD4 + CD25 + cells from non-allergic controls were able to suppress influenza antigen and birch pollen stimulated effector cell proliferation, whereas CD4 + CD25 + cells from allergic patients suppressed influenza antigen-, but not birch pollen-stimulated proliferation. The production of Th1 cytokines, but not Th2 cytokines, was suppressed by CD4 + CD25 + cells from both allergic patients and controls, upon stimulation with birch pollen extract. Neutralization of IL-10 led to significantly increased production of IFN-γ in cultures with CD4 + CD25 - T effector cells. In addition, six-fold higher concentrations of TNF-α were detected after neutralization of IL-10 in both CD4 + CD25 - and CD4 + CD25 + cell cultures from allergic patients and controls. Conclusion We demonstrate that the allergen-specific suppressive function of CD4 + CD25 + cells from allergic patients is impaired compared with non-allergic controls. Moreover, neutralization of IL- 10 enhances the production of TNF-α, suggesting counter-acting properties of IL- 10 and TNF-α, where IL-10 promotes tolerance and suppression by Treg cells and TNF-α promotes inflammatory responses. |
| Databáze: | OpenAIRE |
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