Anti-Proliferative, Analgesic and Anti-Inflammatory Properties of Syzygium mundagam Bark Methanol Extract

Autor: Blassan P. George, Rahul Chandran, Heidi Abrahamse
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Syzygium
Pharmaceutical Science
Pharmacology
030226 pharmacology & pharmacy
Analytical Chemistry
chemistry.chemical_compound
Mice
0302 clinical medicine
Adenosine Triphosphate
Drug Discovery
Edema
pain
Analgesics
Chemistry
Anti-Inflammatory Agents
Non-Steroidal
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Toxicity
MCF-7 Cells
Plant Bark
Molecular Medicine
Female
medicine.symptom
medicine.drug_class
inflammatory mediators
Analgesic
Inflammation
Hoechst stain
Anti-inflammatory
Article
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
In vivo
Lactate dehydrogenase
medicine
Toxicity Tests
Acute
Animals
Humans
granuloma tissue
Physical and Theoretical Chemistry
Rats
Wistar
Cell Proliferation
Plants
Medicinal
L-Lactate Dehydrogenase
Plant Extracts
Methanol
Organic Chemistry
lactate dehydrogenase
Antineoplastic Agents
Phytogenic
Carrageenan
Zdroj: Molecules, Vol 25, Iss 2900, p 2900 (2020)
Molecules
Volume 25
Issue 12
ISSN: 1420-3049
Popis: Cancer, pain and inflammation have long been a cause for concern amongst patients, clinicians and research scientists. There is an alarming increase in the demand for medicines suppressing these disease conditions. The present study investigates the role of Syzygium mundagam bark methanol (SMBM) extract against MCF-7 breast cancer cells, pain and inflammation. The MCF-7 cells treated with SMBM were analyzed for adenosine triphosphate (ATP), lactate dehydrogenase (LDH) levels, changes in cell morphology and nuclear damage. Hot plate, acetic acid and formalin-induced pain models were followed to determine the analgesic activity. Anti-inflammatory activity was studied using carrageenan, egg albumin and cotton pellet induced rat models. Microscopic images of cells in SMBM treated groups showed prominent cell shrinkage and nuclear damage. Hoechst stain results supported the cell death morphology. The decline in ATP (47.96%) and increased LDH (40.96%) content indicated SMBM induced toxicity in MCF-7 cells. In the in vivo study, a higher dose (200 mg/kg) of the extract was found to be effective in reducing pain and inflammation. The results are promising and the action of the extract on MCF-7 cells, pain and inflammation models indicate the potential of drugs of natural origin to improve current therapies.
Databáze: OpenAIRE
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