A novel p38 MAPK gene in the sea cucumber Apostichopus japonicus (Ajp38) is associated with the immune response to pathogenic challenge
| Autor: | Yaqing Chang, Jian Song, Yaoyao Zhan, Kaiquan Li, Yi Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway DNA Complementary Aquatic Science p38 Mitogen-Activated Protein Kinases Transcriptome 03 medical and health sciences Sea cucumber 0302 clinical medicine Rapid amplification of cDNA ends Complementary DNA Animals Environmental Chemistry RNA Messenger Cloning Molecular Gene Vibrio biology Sequence Analysis DNA General Medicine biology.organism_classification Up-Regulation Cell biology Open reading frame 030104 developmental biology Stichopus 030220 oncology & carcinogenesis Apostichopus japonicus |
| Zdroj: | Fish & Shellfish Immunology. 81:250-259 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2018.07.001 |
| Popis: | The p38 mitogen-activated protein kinase (MAPK), an important component of the MAPK signal cascade, is activated by extracellular stimuli, such as environmental stress and pathogenic infection. To clarify the function of p38 MAPKs in echinoderms, we used transcriptome database mining and rapid amplification of cDNA ends (RACE) to identify a novel p38 MAPK gene in the sea cucumber Apostichopus japonicus (here designated Ajp38). The full-length cDNA of Ajp38 was 2231 bp, including an open reading frame encoding 356 amino acid residues. Our sequence analysis indicated that the predicted Ajp38 protein contained the dual phosphorylation site Thr-Gly-Tyr (TGY) and was similar to the p38 homolog in sea urchins. Quantitative real-time PCR analysis showed that Ajp38 was ubiquitously expressed in all examined tissues of healthy adult A. japonicus, with the highest level of expression identified in the coelomocytes. Ajp38 mRNA expression was significantly upregulated in the coelomocytes 4, 12, and 72 h post in vivo infection with Vibrio splendidus. Our results provide more information about the characteristics and immune functions of the p38 homolog in sea cucumbers. |
| Databáze: | OpenAIRE |
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