Isolation and Characterization of Matrix Associated Region DNA Fragments in Rice (Oryza sativa L.)
| Autor: | Wataru Saito, Hiromitsu Moriyama, Kiyoshi Masuda, Masayasu Inoue, Koji Nomura, Seiko Takahashi, Kimiyo Ono |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Plant Physiology Molecular Sequence Data EcoRI Plant Science Biology chemistry.chemical_compound medicine Nuclear Matrix Cloning Molecular Nuclear protein Scaffold/matrix attachment region Plant Proteins Genetics Oryza sativa Base Sequence Cell Membrane Nucleic acid sequence Nuclear Proteins food and beverages Oryza Cell Biology General Medicine Nuclear matrix Blotting Southern Cell nucleus medicine.anatomical_structure chemistry Biochemistry biology.protein Electrophoresis Polyacrylamide Gel Genome Plant DNA |
| Zdroj: | Plant and Cell Physiology. 38:1060-1068 |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | To investigate the interactions between chromosomal DNA and nuclear matrices in higher plants, matrix associated regions (MARs) of rice (Oryza sativa L.) DNAs were cloned. First, we prepared nuclear matrices from isolated nuclei by digesting them with EcoRI and then extracting with 2 M NaCl. About 6% of the total DNA remained in the nuclear matrices after this digestion and extraction. The residual DNA fragments in the nuclear matrices were cloned. Some of the cloned DNA fragments showed binding to certain nuclear proteins. One of the MAR fragments contained sequences related to known consensus motifs and a hairpin loop structure. A method is presented for isolation of matrix associated region (MAR) DNAs from plant cells. |
| Databáze: | OpenAIRE |
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