Expression of programmed cell death protein 1 and T‐cell immunoglobulin‐ and mucin‐domain‐containing molecule‐3 on peripheral blood CD4+CD8+ double positive T cells in patients with chronic hepatitis C virus infection and in subjects who spontaneously cleared the virus
| Autor: | Agnieszka Pawełczyk, Sylwia Osuch, Karol Perlejewski, Maciej Janiak, Kamila Caraballo Cortés, Justyna Kaźmierczak, Khalil Nazzal, Tomasz Laskus, Marek Radkowski, Joanna Jabłońska, Iwona Bukowska-Ośko, Hanna Berak, Marcin Paciorek, Anna Stelmaszczyk-Emmel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
hepatitis C virus
Adult CD4-Positive T-Lymphocytes Male T cell Hepatitis C virus Programmed Cell Death 1 Receptor CD8-Positive T-Lymphocytes medicine.disease_cause Peripheral blood mononuclear cell Epitope 03 medical and health sciences 0302 clinical medicine Immune system T-Lymphocyte Subsets Virology MHC class I medicine Humans 030212 general & internal medicine Hepatitis A Virus Cellular Receptor 2 Aged Hepatology biology business.industry PD‐1 Original Articles Hepatitis C Chronic Middle Aged Tim‐3 Infectious Diseases medicine.anatomical_structure DP T cells Immunology biology.protein Leukocytes Mononuclear 030211 gastroenterology & hepatology Original Article Female Antibody business CD8 |
| Zdroj: | Journal of Viral Hepatitis |
| ISSN: | 1365-2893 1352-0504 |
| Popis: | Chronic hepatitis C virus (HCV) infection is characterized by increased proportion of CD4+CD8+ double positive (DP) T cells, but their role in this infection is unclear. In chronic hepatitis C, immune responses to HCV become functionally exhausted, which manifests itself by increased expression of programmed cell death protein 1 (PD‐1) and T‐cell immunoglobulin‐ and mucin‐domain‐containing molecule‐3 (Tim‐3) on T cells. The aim of our study was to determine PD‐1 and Tim‐3 phenotype of DP T cells in subjects with naturally resolved and chronic HCV infection. Peripheral blood mononuclear cells from 16 patients with chronic infection and 14 subjects who cleared HCV in the past were stained with anti‐CD3, anti‐CD4, anti‐CD8, anti‐PD‐1 and anti‐Tim‐3 antibodies and, in 12 HLA‐A*02‐positive subjects, MHC class I pentamer with HCV NS31406 epitope. In chronic and past HCV infection, proportions of total DP T cells and PD‐1+ DP T cells were similar but significantly higher than in healthy controls. DP T cells were more likely to be PD‐1+ than either CD4+ or CD8+ single positive (SP) T cells. HCV‐specific cells were present in higher proportions among DP T cells than among CD8+ SP T cells in both patient groups. Furthermore, while the majority of HCV‐specific DP T cells were PD‐1+, the proportion of HCV‐specific CD8+ T cells which were PD‐1+ was 4.9 and 1.9 times lower (chronic and past infection, respectively). PD‐1 and Tim‐3 were predominantly expressed on CD4highCD8low and CD4lowCD8high cells, respectively, and co‐expression of both markers was uncommon. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |