Evaluation of the botanical origin of black cohosh products by genetic and chemical analyses
| Autor: | Yutaka Takahashi, Takashi Hakamatsuka, Sayaka Masada-Atsumi, Yukihiro Goda, Yukie Kumeta |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cimicifuga
Black cohosh Pharmaceutical Science Plant Roots Japan Species Specificity Tandem Mass Spectrometry Chromatography High Pressure Liquid Pharmacology Plant roots Traditional medicine biology Chemistry Plant Extracts Specific mass Cimicifuga racemosa Cimicifugae rhizoma General Medicine biology.organism_classification Dna identification Japanese Pharmacopoeia Metabolome Drug Contamination Rhizome Actaea Phytotherapy |
| Zdroj: | Biologicalpharmaceutical bulletin. 37(3) |
| ISSN: | 1347-5215 |
| Popis: | Despite the increasing sales of black cohosh (the dried rhizome and root of Cimicifuga racemosa L.) in the world herbal market, these products have continuous adulteration issues. The botanical authenticity of the black cohosh products is the first important step for ensuring their quality, safety and efficacy. In this study, we genetically identified the botanical sources of 10 black cohosh products and 5 Cimicifuga Rhizome crude drugs of Japanese Pharmacopoeia grade, and analyzed the metabolic profiling of 25 black cohosh products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Consequently, we found that C. dahurica and possibly C. foetida are misused as sources of the black cohosh products and in some cases, the extracts of black cohosh were adulterated with the plant materials of C. dahurica. We demonstrated that these three species can be distinguished by three marker compounds in a specific mass range. These results must be helpful in establishing regulations for the safe use of the black cohosh products. |
| Databáze: | OpenAIRE |
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