Evidence of endoplasmic reticulum stress and liver inflammation in the American mink Neovison vison with benign hepatic steatosis
| Autor: | Darya Kryzskaya, Raija Tammi, Vesa Kärjä, Kirsti Rouvinen-Watt, Petteri Nieminen, Timothy Martin, Tessema Astatkie, Anne-Mari Mustonen, Markku Tammi, Lora Harris, Catherine Pal |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Physiology Biology Biochemistry Liver disease Endocrinology Internal medicine biology.animal medicine Animals RNA Messenger American mink Mink Endoplasmic Reticulum Chaperone BiP Ecology Evolution Behavior and Systematics Chemokine CCL2 Heat-Shock Proteins Lipoprotein lipase Tumor Necrosis Factor-alpha Fatty liver medicine.disease biology.organism_classification Endoplasmic Reticulum Stress Fatty Liver Disease Models Animal Lipoprotein Lipase medicine.anatomical_structure Liver Hepatocyte Animal Science and Zoology Female Steatosis Steatohepatitis Food Deprivation |
| Zdroj: | Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 184(7) |
| ISSN: | 1432-136X |
| Popis: | We investigated the presence of inflammatory signs in the progression of fatty liver disease induced by fasting. Sixty standard black American mink (Neovison vison) were fasted for 0, 1, 3, 5, or 7 days and one group for 7 days followed by re-feeding for 28 days. Liver sections were evaluated histologically and liver mRNA levels indicating endoplasmic reticulum (ER) stress, adipogenic transformation, and inflammation were assessed by quantitative real-time PCR. After 3 days of fasting, the mink had developed moderate liver steatosis. Increased hyaluronan reactivity in lymphocytic foci but no Mallory–Denk bodies were seen in livers of the mink fasted for 5–7 days. Up-regulation of glucose-regulated protein, 78 kDa was observed on day 7 indicating ER stress, especially in the females. Liver lipoprotein lipase and monocyte chemoattractant protein 1 mRNA levels increased in response to 5–7 days of food deprivation, while tumor necrosis factor α (TNF-α) was the highest in the mink fasted for 5 days. The expression of the genes of interest, except for TNF-α, correlated with each other and with the liver fat content. The mRNA levels were found to change more rapidly below n-3/n-6 polyunsaturated fatty acid ratio threshold of 0.15. Following re-feeding, hepatocyte morphology and mRNA abundance returned to pre-fasting levels. Within the studied timeframe, evidence for ER stress, adipogenic transformation, and liver inflammation suggested incipient transition from steatosis to steatohepatitis with potential for development of more severe liver disease. This may present a possibility to influence disease progression before histologically observable steatohepatitis. |
| Databáze: | OpenAIRE |
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