Application of β-glucosidase Immobilized on Chitosan microspheres in Degradation of Polydatin in Polygonum cuspidatum
| Autor: | He Li, Jeonyun Yun, Xiong Xiao, Shan Liu, Zujun Deng, Wei Zong |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
lcsh:GE1-350
0303 health sciences Chromatography biology Chemistry 02 engineering and technology Resveratrol 021001 nanoscience & nanotechnology Enzyme assay Chitosan 03 medical and health sciences chemistry.chemical_compound Hydrolysis Adsorption biology.protein Degradation (geology) Glutaraldehyde 0210 nano-technology lcsh:Environmental sciences POLYGONUM CUSPIDATUM 030304 developmental biology |
| Zdroj: | E3S Web of Conferences, Vol 233, p 02034 (2021) |
| ISSN: | 2267-1242 |
| DOI: | 10.1051/e3sconf/202123302034 |
| Popis: | Resveratrol in Polygonum cuspidatum is a β-glycoside, which can be hydrolyzed to resveratrol by β-glucosidase. it is an efficient production process to degrade polydatin from Polygonum cuspidatum extract by immobilized β-glucosidase. It is of great significance to explore suitable immobilization conditions to improve the catalytic efficiency and reusability of β-glucosidase for polydatin degradation and cost reduction. In this paper, the recombinant Escherichia coli bgl2238, which was screened and constructed from corn soil of Heilongjiang Province in the early laboratory, was immobilized by chitosan adsorption and glutaraldehyde crosslinking. The preparation conditions and immobilization process of bgl2238 were determined by single factor method: the optimal crosslinking time was 1 h, the optimal crosslinking temperature was 20 °C, the recovery rate of enzyme activity of bgl2238 was 87 %, and the enzyme activity was 859.65 mU/g. The optimum temperature of the immobilized bgl2238 is 50 °C, which is 6 °C higher than that of the free bgl2238, and the temperature stability and pH stability are improved. After six consecutive hydrolysis of Polygonum cuspidatum, the degradation rate of polydatin is still over 70 %, which proves that the immobilized bgl2238 has good reusability. This will be helpful to evaluate the application prospect of β - glucosidase immobilized in this system and determine the best conditions for its production. |
| Databáze: | OpenAIRE |
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