Multinode Acoustic Focusing for Parallel Flow Cytometry
| Autor: | Gabriel P. López, Travis A. Woods, Menake E. Piyasena, Steven W. Graves, Pearlson P. Austin Suthanthiraraj, Andrew M. Goumas, Robert W. Applegate |
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| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Erythrocytes Parallel flow medicine.diagnostic_test Chemistry Nanotechnology Acoustics Microfluidic Analytical Techniques Flow Cytometry Article Microspheres Immunophenotyping Analytical Chemistry Volumetric flow rate Flow cytometry Microsphere Flow (mathematics) Erythrocyte Count medicine Humans Throughput (business) Cytometry Biomedical engineering |
| Zdroj: | Analytical Chemistry. 84:1831-1839 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Flow cytometry can simultaneously measure and analyze multiple properties of single cells or particles with high sensitivity and precision. Yet, conventional flow cytometers have fundamental limitations with regards to analyzing particles larger than about 70 microns, analyzing at flow rates greater than a few hundred microliters per minute, and providing analysis rates greater than 50,000 per second. To overcome these limits, we have developed multi-node acoustic focusing flow cells that can position particles (as small as a red blood cell and as large as 107 microns in diameter) into as many as 37 parallel flow streams. We demonstrate the potential of such flow cells for the development of high throughput, parallel flow cytometers by precision focusing of flow cytometry alignment microspheres, red blood cells, and the analysis of CD4+ cellular immunophenotyping assay. This approach will have significant impact towards the creation of high throughput flow cytometers for rare cell detection applications (e.g. circulating tumor cells), applications requiring large particle analysis, and high volume flow cytometry. |
| Databáze: | OpenAIRE |
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