R5 HIV env and vesicular stomatitis virus G protein cooperate to mediate fusion to naive CD4+ T Cells
| Autor: | Una O'Doherty, Matthew J. Pace, Luis M. Agosto |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Receptors CCR5 viruses Immunology Vesicular stomatitis Indiana virus Microbiology Membrane Fusion Virus Viral Envelope Proteins Virology Humans Mononegavirales Membrane Glycoproteins biology Virion env Gene Products Human Immunodeficiency Virus Lipid bilayer fusion virus diseases Rhabdoviridae Virus Internalization biology.organism_classification Fusion protein Virus-Cell Interactions Vesicular stomatitis virus Insect Science Lentivirus HIV-1 |
| Zdroj: | Journal of virology. 85(1) |
| ISSN: | 1098-5514 |
| Popis: | Naïve CD4 4 T cells are resistant to both HIV R5 env and vesicular stomatitis virus G protein (VSV-G)-mediated fusion. However, viral particles carrying both HIV R5 env and VSV-G infect naïve cells by an unexplained mechanism. We show that VSV-G-pseudotyped virus cannot fuse to unstimulated cells because the viral particles cannot be endocytosed. However, virions carrying both HIV R5 env and VSV-G can fuse because CD4 binding allows viral uptake. Our findings reveal a unique mechanism by which R5 HIV env and VSV-G cooperate to allow entry to naïve CD4 + T cells, providing a tool to target naïve CD4 + T cells with R5 HIV to study HIV coreceptor signaling and latency. |
| Databáze: | OpenAIRE |
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