Steady-state EB cap size fluctuations are determined by stochastic microtubule growth and maturation
| Autor: | Thomas Surrey, Jamie Rickman, Lewis D. Griffin, Nicholas I. Cade, Christian Duellberg |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Multidisciplinary Steady state Kinetics Biology Biological Sciences Instability Guanosine Diphosphate Microtubules Quantitative Biology::Subcellular Processes 03 medical and health sciences Crystallography 030104 developmental biology Amplitude Microtubule Tubulin Biophysics Humans Guanosine Triphosphate Growth speed Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 114(13) |
| ISSN: | 1091-6490 |
| Popis: | Growing microtubules are protected from depolymerization by the presence of a GTP or GDP/Pi cap. End-binding proteins of the EB1 family bind to the stabilizing cap, allowing monitoring of its size in real time. The cap size has been shown to correlate with instantaneous microtubule stability. Here we have quantitatively characterized the properties of cap size fluctuations during steady-state growth and have developed a theory predicting their timescale and amplitude from the kinetics of microtubule growth and cap maturation. In contrast to growth speed fluctuations, cap size fluctuations show a characteristic timescale, which is defined by the lifetime of the cap sites. Growth fluctuations affect the amplitude of cap size fluctuations; however, cap size does not affect growth speed, indicating that microtubules are far from instability during most of their time of growth. Our theory provides the basis for a quantitative understanding of microtubule stability fluctuations during steady-state growth. |
| Databáze: | OpenAIRE |
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