Enhancement of cardiomyogenesis in stem cells by low intensity pulsed ultrasound
| Autor: | Yufeng Zhou, Ailing Teo, Jen-Chieh Wang, Mayasari Lim, Amir Morshedi |
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| Přispěvatelé: | School of Chemical and Biomedical Engineering, School of Mechanical and Aerospace Engineering, Proceedings from the 14th International Symposium on Therapeutic Ultrasound |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Angiotensin II receptor type 1
Regeneration (biology) Cartilage Stem Cells Engineering::Bioengineering [DRNTU] Low-intensity pulsed ultrasound Biology Embryonic stem cell Molecular biology Angiotensin II Cell biology medicine.anatomical_structure Low Intensity Pulsed Ultrasound medicine Stem cell Mechanotransduction |
| Popis: | Low intensity pulsed ultrasound (LIPUS) has been shown to enhance bone and cartilage regeneration from stem cells. Gene expression of angiotensin II type 1 (AT1) receptor can be increased in LIPUS-treated osteoblasts. The AT1 receptor is a known mechanoreceptor in cardiomyocytes. It suggests that LIPUS may enhance cardiomyogenesis via mechanotransduction by increasing AT1 expression. Murine embryonic stem cells (ESCs) were treated daily by 10-min 1MHz LIPUS at spatial-average temporal-peak acoustic intensities of 30 mW/cm2 and 300 mW/cm2 in both continuous and pulsed wave (20% duty cycle) for 10 days. Polymerase chain reaction (PCR), immunocytochemistry, and beating rate were used to evaluate the cardiac viability quantitatively. After the treatment of LIPUS, beating rate of contractile areas and cardiac gene expression, such as α- and β-myosin heavy chain, were improved. Furthermore, no deleterious effects to the development of cardiac proteins were observed. All results suggest that LIPUS stimulation has the capacity of enhancing cardiomyogenesis from embryonic stem cells. With the benefit and the ease in incorporating LIPUS into various culture platforms, LIPUS has the potential to produce cardiomyocytes for clinical use in the future. Published version |
| Databáze: | OpenAIRE |
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