Comparative sequence analysis of B5R gene of zoonotic buffalo pox virus isolates with other orthopoxviruses
| Autor: | C. S. Chethana, Mahusudan Hosamani, B. M. Chandranaik, Raj Kumar Singh, Giriappa Krishnappa, C. Renukaprasad, Balur R. Harish |
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| Rok vydání: | 2010 |
| Předmět: |
Buffaloes
Sequence analysis viruses Molecular Sequence Data India Chick Embryo Orthopoxvirus Poxviridae Infections Biology Skin Diseases DNA sequencing Virus Disease Outbreaks law.invention Viral Envelope Proteins Food Animals law Zoonoses Chlorocebus aethiops Parenchyma Animals Humans Amino Acid Sequence Vero Cells Gene Polymerase chain reaction Isolation (microbiology) Virology Vero cell Cattle Animal Science and Zoology |
| Zdroj: | Tropical Animal Health and Production. 43:287-290 |
| ISSN: | 1573-7438 0049-4747 |
| Popis: | The present paper describes the isolation of buffalo pox virus from scab lesions and its molecular characterization through B5R gene sequencing. During our study, pustular pox lesions were observed on the teats and mammary parenchyma of cattle and buffaloes, and the disease was of significant zoonotic importance since similar lesions were produced on the hands, legs, and face of people in close contact with the affected animals. The collected scab materials were subjected for virus isolation in 9-11-day-old chicken embryos by the chorioallontoic membrane route and in the Vero cell line. The virus was confirmed by a sensitive and rapid diagnostic polymerase chain reaction using the primers that amplify "A type inclusion" gene, and further, B5R gene of the virus was sequenced and compared with the corresponding sequences of other orthopoxviruses. The results showed high sequence homology of our isolates with other orthopoxviruses. |
| Databáze: | OpenAIRE |
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