Retinoic acid induces a tissue-specific deletion in the expression domain of Otx2
| Autor: | Frédéric Clotman, G. Van Maele-Fabry, J.J. Picard |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
EMX2
Retinoic acid Mice Inbred Strains Tretinoin In situ hybridization Biology Toxicology Midbrain Embryonic and Fetal Development Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Prosencephalon Developmental Neuroscience Mesencephalon Gene expression Image Processing Computer-Assisted Animals Gene In Situ Hybridization Genetics Neural fold Gene Expression Regulation Developmental Reproducibility of Results Embryo Cell biology chemistry Organ Specificity Gene Deletion |
| Zdroj: | Neurotoxicology and Teratology. 19:163-169 |
| ISSN: | 0892-0362 |
| Popis: | The expression domain of Otx2, a gene essential for the development of the fore- and midbrain, has previously been shown to be affected by exposure to all-trans-retinoic acid (AT-RA). However, morphological abnormalities of the fore- and midbrain induced by exposure of early somite-stage embryos to AT-RA were not associated with abnormal Otx2 expression. To identify abnormal expression of developmental genes induced by exposure at early somite-stages, we performed a fine analysis of the expression domains of Otx2, Otx1, Emx2, and Pax-6 by combining in situ hybridization (ISH) with computer-assisted superpositions and three-dimensional reconstructions of these expression domains. No alteration in the relative location of the caudal boundaries of the expression domains of these genes was observed. The only abnormality was a deletion of the most cranial portion of the neural folds (NF). |
| Databáze: | OpenAIRE |
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