An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings
| Autor: | Stefan de Folter, Elena R. Alvarez-Buylla, Gerco C. Angenent, John Franken, Karla L. Gonzalez-Aguilera, Nayelli Marsch-Martínez |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
ft protein
food.ingredient shoot Sinapis Plant Science Biology cotyledon Pisum Sativum food Arabidopsis Botany Genetics BIOS Plant Development Systems plants Methodology food and beverages biology.organism_classification root PRI Bioscience rnas Horticulture Shoot transport movement Rootstock Cotyledon White mustard Biotechnology |
| Zdroj: | Plant Methods, 9 Plant Methods 9 (2013) Plant Methods |
| ISSN: | 1746-4811 |
| Popis: | Background: Grafting procedures are an excellent tool to study long range signalling processes within a plant. In the last decade, suitable flat-surface grafting procedures for young Arabidopsis seedlings using a collar to support the graft have been developed, allowing the study of long-range signals from a molecular perspective. Results: In the modification presented here, scion and stock are put together on the medium without supporting elements, while cotyledons are removed from the scion, resulting in increased grafting success that can reach up to 100%. At the same time, the protocol enables to process as many as 36 seedlings per hour, which combined with the high success percentage represents increased efficiency per time unit. Conclusions: Growing cotyledons usually push the scion and the rootstock away in the absence of a supporting element. Removing them at the grafting step greatly improved success rate and reduced post-grafting manipulations. Background Grafting procedures have been commonly and successfully used in many plant species for agricultural purposes since ancient times, and are also an excellent tool to study long range signalling processes within plants. The ability to use chimaeric plants can be instrumental for addressing questions concerning non cell-autonomous processes and long-range signal movement within plants. Elegant classic grafting experiments in plants like pea (Pisum sativum), white mustard (Sinapis alba) and other species have enriched the understanding of processes such as flowering time and hormone signalling, among others. In the last decade, suitable grafting protocols have also been developed for the model plant Arabidopsis thaliana, allowing the study of long range signals from a molecular |
| Databáze: | OpenAIRE |
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