Degradation Products of Tryptophan in Cell Culture Media: Contribution to Color and Toxicity
| Autor: | Aline Zimmer, Anton Lindig, Alisa Schnellbaecher, Brit Pardon, Maxime Le Mignon, Tim Hofmann, Stephanie Bellmaine |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Antioxidant antioxidant biomanufacturing QH301-705.5 medicine.medical_treatment CHO Cells 01 natural sciences Redox Article Catalysis Inorganic Chemistry Ammonia production Cricetulus 010608 biotechnology Browning medicine Animals Biomanufacturing tryptophan Food science Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy 010405 organic chemistry Chemistry Organic Chemistry Tryptophan General Medicine 0104 chemical sciences Computer Science Applications Culture Media color LC-MS cell culture media Toxicity Degradation (geology) cytotoxicity Oxidation-Reduction |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 6221, p 6221 (2021) International Journal of Molecular Sciences Volume 22 Issue 12 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Biomanufacturing processes may be optimized by storing cell culture media at room temperature, but this is currently limited by their instability and change in color upon long-term storage. This study demonstrates that one of the critical contributing factors toward media browning is tryptophan. LC-MS technology was utilized to identify tryptophan degradation products, which are likely formed primarily from oxidation reactions. Several of the identified compounds were shown to contribute significantly to color in solutions but also to exhibit toxicity against CHO cells. A cell-culture-compatible antioxidant, a-ketoglutaric acid, was found to be an efficient cell culture media additive for stabilizing components against degradation, inhibiting the browning of media formulations, and decreasing ammonia production, thus providing a viable method for developing room-temperature stable cell culture media. |
| Databáze: | OpenAIRE |
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