Fungal extracts detected in eosinophilic chronic rhinosinusitis induced cytokines from the nasal polyp cells
| Autor: | Ken Kikuchi, Noritsugu Ono, Masato Miwa, Akihito Shiozawa, Mikio Hirotsu, Katsuhisa Ikeda |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty polymerase chain reaction medicine.medical_treatment Proinflammatory cytokine Young Adult Nasal Polyps Eosinophilia Eosinophilic cytokine otorhinolaryngologic diseases Humans Medicine eosinophil Nasal polyps Prospective Studies Sinusitis Allergy/Rhinology Aged Rhinitis nasal polyp Fungus business.industry Fungi Middle Aged respiratory system Eosinophil medicine.disease Fungal antigen medicine.anatomical_structure Cytokine Otorhinolaryngology Chronic Disease Cytokines Female medicine.symptom business |
| Zdroj: | The Laryngoscope |
| ISSN: | 1531-4995 0023-852X |
| DOI: | 10.1002/lary.24655 |
| Popis: | Objectives/Hypothesis The role of fungi in chronic rhinosinusitis (CRS) is still controversial. The present study was conducted to detect and identify fungal species from the nasal polyp tissues of eosinophilic and noneosinophilic CRS, and to determine the role of fungal antigens in cytokine production. Study Design Prospective study. Methods Thirty-five specimens of nasal polyps were collected from patients with CRS and examined for fungus using culture, histology, and polymerase chain reaction analysis. The secretion of 14 cytokines stimulated by fungal extracts using dispersed nasal polyp cells (DNPCs) was determined by multiplex immunoassay. Results There was no microbiological growth (including fungus) in the cultures of homogenized nasal polyps. Furthermore, Grocott methanamine silver staining for all nasal polyps showed no fungal bodies. Sixteen of 35 samples of the nasal polyps showed amplification of fungal DNA. In none of the mucosa of the sphenoid sinus was fungal DNA detected. The number of eosinophils in the nasal polyps in which fungal DNA was detected was significantly higher than in the nasal polyps in which fungal DNA was not detected (P |
| Databáze: | OpenAIRE |
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