Preserved extracellular matrix components and retained biological activity in decellularized porcine mesothelium
| Autor: | Chris M. Bowley, Craig M. Neville, Elisabeth M. O’Doherty, David M. Hoganson, Dina O. Harilal, Russell T. Kronengold, Scott M. Goldman, Gwen E. Owens, Joseph P. Vacanti |
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| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Swine Biophysics Biocompatible Materials Enzyme-Linked Immunosorbent Assay Bioengineering Matrix (biology) Fibroblast growth factor Epithelium Cell Line Biomaterials Extracellular matrix Dogs Peritoneum Laminin medicine Animals Humans Glycosaminoglycans Decellularization biology Chemistry Extracellular Matrix Cell biology Fibronectin Mesothelium medicine.anatomical_structure Mechanics of Materials Culture Media Conditioned Microscopy Electron Scanning Ceramics and Composites biology.protein Cytokines |
| Zdroj: | Biomaterials. 31:6934-6940 |
| ISSN: | 0142-9612 |
| Popis: | Mesothelium tissues such as peritoneum and pleura have a thin and strong layer of extracellular matrix that supports mesothelial cells capable of rapid healing. Decellularized porcine mesothelium was characterized for strength, composition of the matrix and biological activity. The tensile strength of the material was 40.65 +/- 21.65 N/cm. Extracellular matrix proteins collagen IV, fibronectin, and laminin as well as glycosaminoglycans were present in the material. Cytokines inherent in the extracellular matrix were preserved. Vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and transforming growth factor beta (TGF-beta) were retained and the levels of VEGF and TGF-beta in the decellularized mesothelium were higher than those found in decellularized small intestinal submucosa (SIS). The decellularized mesothelium also stimulated human fibroblasts to produce more VEGF than fibroblasts grown on tissue culture plastic. Decellularized mesothelium is a sheet material with a combination of strength and biological activity that may have many potential applications in surgical repair and regenerative medicine. |
| Databáze: | OpenAIRE |
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