Alterations in levels of 8-Oxo-2 '-deoxyguanosine and 8-Oxoguanine DNA glycosylase 1 during a current episode and after remission in unipolar and bipolar depression
| Autor: | Melis Kant, Selda Yılmaz, Marin Veldic, Burcu Verim, Pinar Akan, Hüray İşlekel, Ayse Er, Deniz Ceylan, Gamze Tuna, Ayşegül Özerdem, Ayşegül Ildız, Mark A. Frye, Merve Akış |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty Bipolar Disorder DNA repair DNA damage Endocrinology Diabetes and Metabolism Gene Expression 8-Oxo-2'-deoxyguanosine Urine Gastroenterology DNA Glycosylases Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine mental disorders Gene expression medicine Humans Biological Psychiatry Depressive Disorder Endocrine and Autonomic Systems business.industry medicine.disease 030227 psychiatry Oxidative Stress Psychiatry and Mental health Mood disorders chemistry 8-Hydroxy-2'-Deoxyguanosine DNA glycosylase Female sense organs business Body mass index 030217 neurology & neurosurgery |
| Popis: | Introduction Previous studies showed significant increases in DNA base damage markers and significant alterations in base excision repair enzymes in patients with unipolar and bipolar depression. We aimed to investigate changes in urine 8-Oxo-2′-deoxyguanosine (8-oxo-dG) and gene expression levels of 8-Oxoguanine DNA glycosylase 1 (OGG1) during a current depressive episode and after remission in bipolar and unipolar disorders. Methods Twenty-four acutely depressed bipolar (BD), 33 unipolar depression (UD) patients and 61 healthy controls were included in the study. Clinical evaluations, blood and urine sampling were completed at baseline and at remission after eight weeks. The urine 8-oxo-dG levels were assessed by liquid chromatography tandem mass spectrometry and adjusted for urine creatinine levels. The gene expression levels of OGG1 were determined from cDNA extracted from blood samples, using real time-polymerase chain reaction. Results At baseline, patients presented significantly higher levels of 8-oxo-dG (p = 0.008), and lower gene expression of OGG1 (p = 0.024) compared to controls. Levels of either 8-oxo-dG or OGG1 expression did not differ between BD and UD. In patients who remitted by the 8th week (n = 30), 8-oxo-dG decreased significantly (p = 0.001), and gene expression levels of OGG1 increased by 2.95 times compared to baseline levels (p = 0.001). All comparisons were adjusted for age, sex, smoking status and body mass index. Conclusion Our results suggest that patients with bipolar and unipolar mood disorders present increased 8-oxo-dG and decreased gene expression levels of OGG1 in current depressive episodes, and that these changes might be reversed by the resolution of depressive symptoms. The causal relationship between DNA damage and repair requires further exploration. |
| Databáze: | OpenAIRE |
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