New insights into sugar characterization in complex plant matrices by high-performance thin-layer chromatography
| Autor: | Tatiana Bernardi, Silvia Vertuani, Daniela De Lucia, Stefano Manfredini |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
fiber analysis
Clinical Biochemistry Pharmaceutical Science Adansonia digitata food oligosaccharides HPLC HPTLC sugar characterization Analytical Chemistry Biochemistry 3003 01 natural sciences High-performance liquid chromatography chemistry.chemical_compound 0404 agricultural biotechnology Column chromatography Acetone High performance thin layer chromatography Acetonitrile Derivatization Chromatography Silica gel 010401 analytical chemistry Ambientale 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences chemistry Ultrapure water |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 39:607-612 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | High-performance thin-layer chromatography (HPTLC) is an innovative, green, reliable, and rapid method for the characterization of complex sugar mixtures. For research into sustainable nontimber forest products, Adansonia digitata dry fruit pulp in this proof-of-concept study was used to demonstrate this method due to its reported high nutritional value and prebiotic activity. Chromatographic separations were performed on (20 × 10 cm2) HPTLC glass plates with diol stationary phase, using a 15-step gradient by mixing solutions acetone/acetonitrile 1:1, and ultrapure water for enzymatic degradation products. Instead, monosaccharide moieties were separated on HPTLC silica gel 60 F254 over isocratic mode with 80 mm of eluent front composed of acetonitrile/acetic acid/water 63/33/5 v/v/v. Identification and quantification were performed by densitometry acquisitions at 400 nm after opportune derivatization. Our research highlighted that this technique can be used as a standard method to gain new insight... |
| Databáze: | OpenAIRE |
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