Characterization and measurement of anti-IgG antibodies in human sera by radioimmunoassay (RIA)
| Autor: | David W. Knutson, H. Nasu, David Chia, J. Yamagata, E.V. Barnett |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
biology
Chemistry Immunology Antibody Affinity Radioimmunoassay chemical and pharmacologic phenomena Molecular biology Antibodies Anti-Idiotypic Polyethylene Glycols Latex fixation test Arthritis Rheumatoid Solubility Immunoglobulin G Direct binding biology.protein Chemical Precipitation Humans Immunology and Allergy Indicators and Reagents Polychondritis Relapsing Antibody Latex Fixation Tests Low avidity |
| Zdroj: | Journal of Immunological Methods. 29:43-56 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(79)90124-8 |
| Popis: | A sensitive direct binding radioimmunoassay (RIA) was developed which detected low avidity anti-IgG antibodies in sera negative in the latex fixation test (LFT). IgG class antibodies could be detected and were commonly found along with IgM class antibodies. Additionally, the RIA was more reproducible than the LFT, was easily adapted to measure relative avidities of anti-IgG antibodies, and had other technical advantages over the LFT. |
| Databáze: | OpenAIRE |
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