Expression of neuregulin 4 splice variants in normal human tissues and prostate cancer and their effects on cell motility
| Autor: | Teresa M. Frost, Byron J. T. Morgan, Mary M Boyle, Graham A. Russell, Nandini V. L. Hayes, Edith Blackburn, William J. Gullick |
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| Rok vydání: | 2010 |
| Předmět: |
Gene isoform
Male Cancer Research Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism Cell Motility Biology Adenocarcinoma Transfection Mice Endocrinology Cell Movement Chlorocebus aethiops medicine Animals Humans Protein Isoforms education Receptor ERBB4 Neuregulins Neuregulin-4 education.field_of_study Principal Component Analysis Alternative splicing Prostatic Neoplasms Prostate-Specific Antigen Cell biology Alternative Splicing medicine.anatomical_structure Oncology COS Cells NIH 3T3 Cells |
| Zdroj: | Endocrine-related cancer. 18(1) |
| ISSN: | 1479-6821 |
| Popis: | The neuregulin 4 gene encodes at least five different variants (designated A1, A2, B1, B2 and B3) produced as a result of alternative splicing. We have determined their sites of expression in normal human adult tissues using isoform-specific antibodies. Their expression is cell type specific and differs in subcellular location suggesting that they may have varied functions in these contexts. We have shown in a panel of prostate cancers that each form is present to differing degrees, and that principal component analysis indicates that there are three patterns of expression. Some isoforms were positively correlated with high prostate-specific antigen levels and others were inversely associated with Gleason score. Synthetic, refolded A forms promoted lamellipodia and filopodia formation in cells expressing the ErbB4 (CTa) receptor and stimulated cell motility in wound healing assays. The data suggest that the different forms have varied sites of expression and function, and this includes effects on cell architecture and motility. |
| Databáze: | OpenAIRE |
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