Do platelet apoptosis, activation, aggregation, lipid peroxidation and platelet–leukocyte aggregate formation occur simultaneously in hyperlipidemia?
| Autor: | Derya Özsavcı, Rabia Oba, Azize Şener, Gulderen Yanikkaya Demirel, K. Turay Yardimci, Fikriye Uras |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Platelet Aggregation P-selectin Clinical Biochemistry Apoptosis Hyperlipidemias Phosphatidylserines Fibrinogen Lipid peroxidation chemistry.chemical_compound High-density lipoprotein Reference Values Internal medicine Hyperlipidemia Cell Adhesion Leukocytes medicine Humans Platelet Platelet activation Cholesterol General Medicine medicine.disease Glutathione P-Selectin Endocrinology Platelet Glycoprotein GPIb-IX Complex chemistry Immunology Female lipids (amino acids peptides and proteins) Lipid Peroxidation medicine.drug |
| Zdroj: | Clinical Biochemistry. 38:1081-1087 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2005.09.005 |
| Popis: | The circulating lipoproteins may cause some abnormalities in platelet composition and function in hypercholesterolemia. The aim of this study was to investigate whether platelet apoptosis, platelet activation, platelet aggregation, platelet-leukocyte aggregate (PLA) formation and lipid peroxidation occur simultaneously in hyperlipidemia.Expression of GpIIb/IIIa (CD41a), P-selectin (CD62-P), platelet-bound fibrinogen (antifibrinogen), platelet membrane phosphatidylserine (PS), platelet-monocyte aggregates (mono-PLA) and platelet-neutrophil aggregates (neut-PLA) was measured in eight hyperlipidemic and eight normal subjects using flow cytometry. ADP (10 microM) was used to activate platelets. Furthermore, ADP induced platelet aggregation responses, platelet malondialdehyde (MDA) and glutathione (GSH) levels were determined.Before platelet activation, platelet CD62-P, antifibrinogen, annexin-V, mono-PLA, neut-PLA and platelet MDA levels as well as platelet aggregation responses in the hyperlipidemics were significantly higher than those in the controls (P0.01, P0.01, P0.01, P0.001, P0.001, P0.01, P0.001, respectively), whereas GpIIb/IIIa expression and GSH levels were not different significantly (P0.05). In the control group, CD62-P, antifibrinogen and annexin-V levels increased significantly after ADP activation (P0.05, P0.05, P0.01, respectively). In hyperlipidemic subjects, annexin-V expression increased significantly after activation (P0.01), whereas expression of GpIIb/IIIa, CD62-P and antifibrinogen remained unchanged (P0.05). The levels of total cholesterol (T-CHO), low density lipoprotein cholesterol (LDL-C), serum fibrinogen (S-FGN) and high density lipoprotein cholesterol (HDL-C) in patients were found to be correlated with platelet CD62-P, antifibrinogen, annexin-V, mono-PLA and MDA.In conclusion, it seems that in hyperlipidemia, some platelets are in an activated state in circulation, and that increased lipid peroxidation, early apoptosis, platelet-leukocytes aggregate formation and platelet aggregation altogether accompany this process. |
| Databáze: | OpenAIRE |
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