Influence of temperature on the progamic phase in Citrus
| Autor: | María C. Vives, Patrick Ollitrault, José Cuenca, Pablo Aleza, Luis Navarro, Rafael Montalt |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Citrus Gynoecium F60 - Physiologie et biochimie végétale Reproduction sexuée Plant Science Biology medicine.disease_cause 01 natural sciences Plant reproduction 03 medical and health sciences Pollen medicine Ovule Ecology Evolution Behavior and Systematics F63 - Physiologie végétale - Reproduction food and beverages Température Horticulture 030104 developmental biology Germination Pollen tube Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | electronico ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias instname Environmental and Experimental Botany |
| Popis: | Temperature in the progamic phase is critical for the success of plant sexual reproduction, and new knowledge is needed to optimise breeding programmes to obtain new varieties that adapt to a climate change scenario. Using three male donors and one female recipient in the genus Citrus, we evaluated the effect of four temperature regimes on each process in the progamic phase. An innovative method based on microscopic observations of cross sections from pollinated pistils collected daily allowed a comprehensive analysis of pollen tube growth (dynamics and kinetics) along the pistil. Pollen grain germination and stigmatic receptivity were evaluated directly on the stigma, which offers more accurate information than previously reported in vitro experiments. Our results showed that warm temperatures reduce the time needed by pollen tubes to reach ovules and accelerate pistil degeneration, while cold temperatures produce the opposite effects. Interestingly, we observed both pollen grain germination and pollen tube growth at 10 °C, which have not been observed in previous studies in citrus. At this temperature, the differences observed in both pollen grain germination and pollen tube growth for different genotypes reflect the adaptation of their sporophytic generation to low temperatures which would enable gametophytic screening to be used as a tool to select better adapted genotypes to different temperature conditions. The differences observed in the growth rates between pollen tubes in each genotype-temperature combination provide an opportunity to explore additional gametophytic selection in this reproductive phase. The capacity to respond to temperature changes in the progamic phase to ensure mating can be useful for breeding programs that focus on obtaining better adapted populations to different temperature conditions. |
| Databáze: | OpenAIRE |
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