Clinical relevance of genetic instability in prostatic cells obtained by prostatic massage in early prostate cancer
| Autor: | Alain Houlgatte, Karine Chantrel-Groussard, Pierre Teillac, S. Guimard, Philippe Berthon, R. Thuret, Alain Latil, Abdel-Rahmène Azzouzi, Olivier Cussenot, Jean-Marie Villette |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Pathology diagnosis Urinary system Urology Loss of Heterozygosity Sensitivity and Specificity Loss of heterozygosity Prostate cancer localised cancer Prostate Clinical Studies Biopsy medicine Humans LOH Clinical significance RNA Messenger Aged Aged 80 and over Massage medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Prostatic Neoplasms Middle Aged Prostate-Specific Antigen medicine.disease Prostate-specific antigen medicine.anatomical_structure Oncology biomarker Biomarker (medicine) business |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6602311 |
| Popis: | We investigated whether genetic lesions such as loss of heterozygosity (LOH) are detected in prostatic cells obtained by prostatic massage during early diagnosis of prostate cancer (CaP) and discussed their clinical relevance. Blood and first urine voided after prostatic massage were collected in 99 patients with total prostate-specific antigen (PSA) between 4 and 10 ng ml−1, prior to prostate biopsies. Presence of prostatic cells was confirmed by quantitative RT–PCR analysis of PSA mRNA. Genomic DNA was analysed for LOH on six chromosomal regions. One or more allelic deletions were found in prostatic fluid from 57 patients analysed, of whom 33 (58%) had CaP. Sensitivity and specificity of LOH detection and PSA free to total ratio |
| Databáze: | OpenAIRE |
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