Verteporfin photodynamic therapy induced apoptosis in choroidal neovascular membranes
| Autor: | Focke Ziemssen, K. Petermeier, Olcay Tatar, Michael Völker, Karl Ulrich Bartz-Schmidt, Werner Inhoffen, Salvatore Grisanti, Sigrid Henke-Fahle, Silvia Bopp, Faik Gelisken, B. A. Lafaut |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Pathology Porphyrins genetic structures Angiogenesis medicine.medical_treatment Visual Acuity Apoptosis Photodynamic therapy Neovascularization Macular Degeneration Cellular and Molecular Neuroscience In Situ Nick-End Labeling medicine Humans Aged Retrospective Studies Aged 80 and over Retina Photosensitizing Agents TUNEL assay business.industry Clinical Science - Extended Report Verteporfin Middle Aged Macular degeneration medicine.disease Choroidal Neovascularization eye diseases Sensory Systems Surgery Ophthalmology medicine.anatomical_structure Choroidal neovascularization Photochemotherapy Female Endothelium Vascular sense organs medicine.symptom business medicine.drug |
| Zdroj: | British Journal of Ophthalmology. 90:1034-1039 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.2006.090852 |
| Popis: | To evaluate the impact of verteporfin photodynamic therapy (PDT) on the induction of apoptosis in choroidal neovascular membranes (CNV) secondary to age related macular degeneration.Retrospective review of 22 surgically excised CNV. 12 of these patients had been treated with PDT 3-146 days previously. Apoptotic cells were detected with the TUNEL technique and compared to the expression of CD34 (endothelial cells, EC), CD105 (activated endothelial cells), Ki-67 (proliferation marker), and cytokeratin18 (retinal pigment epithelial cells, RPE).CNV excised 3 days after PDT were characterised both by collapsed and patent vessels. The EC displayed a statistical significant positive TUNEL reaction when compared to the remaining treated CNV (p0.001) and untreated CNV (P = 0.002). The proliferative activity was reduced. CNV excised 1-5 months after PDT displayed a patent vascularisation and high proliferative activity. All membranes either treated or untreated disclosed only sporadic TUNEL positive cells within the stroma and the RPE.Verteporfin PDT leads to selective and effective damage of EC within CNV. Both patent and occluded vessels were lined by apoptotic EC. This finding and the increased expression of proliferation marker at later time points suggest that revascularisation after PDT is caused by angiogenesis rather than recanalisation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|