Antioxidant and antimicrobial extracts obtained from agricultural by-products: Strategies for a sustainable recovery and future perspectives

Autor: Silvia Vertuani, Gianni Sacchetti, Massimo Tacchini, Alessandro Grandini, Ilaria Burlini, Alessandra Guerrini, Belén Huerta Lorenzo
Rok vydání: 2020
Předmět:
0106 biological sciences
Antioxidant
DPPH
General Chemical Engineering
medicine.medical_treatment
Cereals
By-products
Bound phenolics
Antioxidant
Antimicrobial
By-products
Cereals
medicine.disease_cause
FA
trans-ferulic acid
01 natural sciences
Biochemistry
Article
UAE
ultrasound-assisted extraction
chemistry.chemical_compound
Economica
0404 agricultural biotechnology
UAAH
ultrasound-assisted alkaline hydrolysis
Staphylococcus epidermidis
010608 biotechnology
medicine
AH
alkaline hydrolysis
Food science
Bound phenolics
Alkaline hydrolysis
ComputingMethodologies_COMPUTERGRAPHICS
ABTS
biology
Chemistry
Extraction (chemistry)
Ambientale
04 agricultural and veterinary sciences
biology.organism_classification
Antimicrobial
040401 food science
Staphylococcus aureus
p-CA
p-coumaric acid
Food Science
Biotechnology
Zdroj: Food and Bioproducts Processing
ISSN: 0960-3085
DOI: 10.1016/j.fbp.2020.10.003
Popis: Graphical abstract
Highlights • Sonication-alkaline hydrolysis combination improve the bound phenolics extraction. • The obtained extracts demonstrated an increased antioxidant activity. • These botanicals were chemically characterized with various approaches. • These extracts are promising ingredient for cosmetic industry.
Durum wheat and rice brans are by-products deriving from the milling industry and source of important phytochemicals, mostly phenolics in bound form. The objectives of this work were to evaluate the most effective method for the extraction of phenolic acids from these by-products with antioxidant and antimicrobial activity and to standardize the obtained extracts to encourage their commercialization in health field. Free phenolics were extracted with ultrasound-assisted extraction (UAE) and bound phenolics with alkaline hydrolysis (AH) and ultrasound-assisted alkaline hydrolysis (UAAH). Extracts were analyzed with HPTLC (high-performance thin-layer chromatography) and RPHPLC-DAD-ESIMS (reversed-phase high-performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry); the content of trans-ferulic acid, p-coumaric acid, total phenolics were also quantified. The antioxidant activity was investigated using DPPH and ABTS methods. The antimicrobial activity was evaluated against Staphylococcus aureus and Staphylococcus epidermidis strains. Results obtained using UAAH showed the most interesting data, suggesting this method as the most effective to obtain active extracts from the by-products. Durum wheat, extracted with UAAH, exhibited the highest content of trans-ferulic acid (406.14 ± 0.65 μg FA/mg extract), total phenolics (610.58 ± 57.60 mg GAE/g dried extract) and good antioxidant and antimicrobial activities and it has been selected to formulate oil-in-water cosmetic products.
Databáze: OpenAIRE
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