Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen
| Autor: | Jin Ma, Xue-Ke Li, Yue Xing, Si-Duo Lu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
collagen
RHOA integrin Integrin opticin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Ophthalmology Medicine ROCK1 Viability assay biology rhoa/rock1 business.industry Cell growth Retinal Adhesion Cell biology Blot Ophthalmology Basic Research chemistry lcsh:RE1-994 030221 ophthalmology & optometry biology.protein retinal vascular endothelial cell business |
| Zdroj: | Int J Ophthalmol International Journal of Ophthalmology, Vol 13, Iss 8, Pp 1202-1209 (2020) |
| ISSN: | 2222-3959 |
| Popis: | AIM: To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells (hRVECs), and explore its regulations by integrins and RhoA/ROCK1 signal pathway. METHODS: hRVECs were cultured in collagen and treated by opticin, and cell-based bioactivity assays of cell proliferation, migration, and adhesion were performed. The expression of integrin α2, integrin β1, RhoA and ROCK1 were examined with real-time PCR and Western blotting. RESULTS: Collagen could promote cell viability of proliferation and migration (all P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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