Multi-biofunctional polymer graphene composite for bone tissue regeneration that elutes copper ions to impart angiogenic, osteogenic and bactericidal properties
| Autor: | Sachin Kumar, L.R. Jaidev, Kaushik Chatterjee |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Thermogravimetric analysis
Bone Regeneration Polymers Polyesters chemistry.chemical_element Nanotechnology macromolecular substances 02 engineering and technology 010402 general chemistry Bone tissue 01 natural sciences Bone and Bones Nanocomposites chemistry.chemical_compound Colloid and Surface Chemistry Osteogenesis medicine Physical and Theoretical Chemistry Bone regeneration Tube formation Nanocomposite technology industry and agriculture Materials Engineering (formerly Metallurgy) Surfaces and Interfaces General Medicine equipment and supplies musculoskeletal system 021001 nanoscience & nanotechnology Exfoliation joint Copper 0104 chemical sciences medicine.anatomical_structure Chemical engineering chemistry Polycaprolactone Graphite 0210 nano-technology Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 159:293-302 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2017.07.083 |
| Popis: | Despite several recent advances, poor vascularization in implanted scaffolds impedes complete regeneration for clinical success of bone tissue engineering. The present study aims to develop a multibiofunctional nanocomposite for bone tissue regeneration using copper nanoparticle decorated reduced graphene oxide (RGO_Cu) hybrid particles in polycaprolactone (PCL) matrix (PCL/RGO_Cu). X-ray photoelectron spectroscopy and X-ray diffraction confirmed the presence of copper oxides (CuO and Cu2O) on RGO. Thermogravimetric analysis showed that 11.8% of copper was decorated on RGO. PCL/RGO_Cu exhibited steady release of copper ions in contrast to burst release from the composite containing copper alone (PCL/Cu). PCL/RGO_Cu exhibited highest modulus due to enhanced filler exfoliation. Endothelial cells rapidly proliferated on PCL/RGO_Cu confirming cytocompatibility. The sustained release of ions from PCL/RGO_Cu composites augmented tube formation by endothelial cells evidenced enhanced angiogenic activity. Gene expression of angiogenic markers VEGF and ANG-2 was higher on PCL/RGO_Cu compared to PCL. The osteogenic activity of PCL/RGO_Cu was confirmed by the 87% increase in mineral deposition by pre-osteoblasts compared to PCL. The bactericidal activity of PCL/RGO_Cu showed 78% reduction in viability of Escherichia colt. Thus, the multi-biofunctional PCL/RGO_Cu composite exhibits angiogenic, osteogenic and bactericidal properties, a step towards addressing some of the critical challenges in bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect