Synthesis, biological evaluations and molecular modelling studies of novel indolin-2-ones designing as FGFR inhibitors

Autor: Fadime Aydın Köse, Gunes Coban
Přispěvatelé: Ege Üniversitesi
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Saudi Pharmaceutical Journal, Vol 27, Iss 7, Pp 952-967 (2019)
Saudi Pharmaceutical Journal : SPJ
ISSN: 1319-0164
3199-7902
Popis: Aydin Kose, Fadime/0000-0001-5222-7555
WOS: 000498856700007
PubMed: 31997902
A series of novel 3,5-disubstituted indolin-2-ones were designed and synthesized as selective FGFR inhibitors. in the design process of 3,5-disubstituted indolin-2-ones for FGFRs, molecular docking studies were performed to generate and optimize novel compounds which have FGFR inhibitory potency, theoretically. in vitro enzyme inhibitory and selectivity profiles of the synthesized compounds, and their cytotoxicity against NIH-3T3 cells were evaluated. According to enzyme inhibition assay, compound A1 (FGFR1-4; IC50 = 19.82; 5.95; 1419; 37150 nM), compound A5 (FGFR1-4; IC50 = 1890; Nd; 6.50; 18590 nM) and compound A13 (FGFR1-4; IC50 = 6.99; 1022; 17090; 8993 nM) have displayed best inhibitory potency against FGFR2, FGFR3 and FGFR1, respectively. the studied compounds have displayed low affinity to FGFR4 in comparison with other isoforms. Molecular docking study data were used to determine the binding orientations of the synthesized compounds inside FGFRs in accordance with enzyme inhibition assay data. Molecular dynamics simulations and free energy calculations were performed to determine stability, binding modes and dynamics behaviors of compound A1, A5 and A13 inside FGFR-2, FGFR-3 and FGFR-1, respectively. the compounds bearing aromatic groups at the C5 position of indolin-2-one could be lead compounds for the development of more effective and selective FGFR1-3 inhibitors. (C) 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University.
Turkish Scientific and Technical Research Council (TUBITAK) (Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115S692]; Ege University (Turkey)Ege University [2015/B_IL/040]
Authors would like to express their sincere thanks toward Turkish Scientific and Technical Research Council (TUBITAK) (Turkey, Grant number 115S692) and Ege University (Turkey, Grant number 2015/B_IL/040), for their financial support, respectively. Authors appreciate to Pharmaceutical Sciences Research Centre (FABAL) of Ege University Faculty of Pharmacy for spectral analyses of the compounds and molecular modelling software support.
Databáze: OpenAIRE
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