Selective Enumeration of Bifidobacterium bifidum , Enterococcus faecium , and Streptomycin-Resistant Lactobacillus acidophilus from a Mixed Probiotic Product
| Autor: | Daryl W. Osato, Connie I. E. Wang, Melissa L. Calicchia, Takehiro Nomura, Fumio Yotsuzuka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Bifidobacterium bifidum biology ved/biology ved/biology.organism_classification_rank.species Streptomycin Sulfate biology.organism_classification Microbiology chemistry.chemical_compound Lactobacillus acidophilus food Enterococcus chemistry Sodium propionate Agar Food science Food Science Enterococcus faecium Bifidobacterium |
| Zdroj: | Journal of food protection. 56(11) |
| ISSN: | 1944-9097 |
| Popis: | Modified VF-Bouillon agar with 0.5 mg/ml lithium chloride, 20 μg/ml sodium lauryl sulfate, 5 mg/ml sodium propionate, and 10 μg/ml neomycin sulfate was used with a triple-layer diffusion technique to selectively enumerate Bifidobacterium bifidum . Modified Brigg's agar was used to enumerate Enterococcus faecium . Modified Brigg's agar with 1,200 μg/ml streptomycin sulfate was used in a double-layer diffusion technique to selectively enumerate a streptomycin-resistant strain of Lactobacillus acidophilus . Selective enumeration of the individual bacterial components was compared to the mixture with an average 99% recovery of each component. |
| Databáze: | OpenAIRE |
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