| Autor: |
Lars Grimelius, Henry Johansson, Allan Bengtsson, Jan Pontén |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Acta Pathologica Microbiologica Scandinavica Section A Pathology. :455-460 |
| ISSN: |
0365-4184 |
| Popis: |
Nuclear DNA-content in parathyroid cell imprints from adenomas, hyperplastic and normal glands was determined. Two principally different staining procedures were applied: paratoseaniline-Feulgen and ethidium-bromide staining. All nuclei, particularly with the ethidium-bromide method were registered as containing even multiples of the normal human diploid DNA-content. Normal and hyperplastic glands showed greater than or equal to 98 per cent diploid nuclei, the remaining fraction was tetraploid. In adenomas the frequency of diploid nuclei varied from 50-98 per cent; the remaining were predominantly tetraploid, but octaploid and higher even ploidity also occurred. A good correlation was found between nuclear DNA-content and nuclear diameter. The findings indicate that determination of nuclear DNA-content of parathyroid cells may be a valuable contribution in the differentiation between hyperplasia and adenoma. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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