Transient expression of murine interferon-alpha genes in mouse and monkey cells
| Autor: | I. Jaap Bosveld, Margreet van Heuvel, Wim Luyten, Ellen C. Zwarthoff, Jan Trapman |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
viruses
Alpha interferon Simian virus 40 Biology Kidney Transfection Cell Line Fusion gene Mice Chlorocebus aethiops Murine leukemia virus Gene expression Escherichia coli Genetics Animals Cloning Molecular Promoter Regions Genetic Enhancer Gene COS cells DNA Restriction Enzymes General Medicine biology.organism_classification Molecular biology Long terminal repeat Genes Interferon Type I Metallothionein Moloney murine leukemia virus Plasmids |
| Zdroj: | Gene. 45:159-165 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(86)90250-7 |
| Popis: | The coding regions of murine interferon-alpha (IFN-alpha) genes were combined with promoter and 3'-noncoding sequences from other eukaryotic genes. Transient expression of these fusion genes was achieved in monkey COS cells and in a mouse cell line (TOP cells) expressing polyoma virus (Py) large T antigen constitutively. The efficiency of the different expression plasmids was determined by measuring the amount of IFN secreted into the medium. Replacement of the 3'-noncoding region of an IFN-alpha gene by that of the rabbit beta-globin gene resulted in a fourfold higher IFN-alpha production. The SV40 early promoter and the Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) produced similar amounts of IFN-alpha in COS cells. However, a tandem combination of the SV40 enhancer/early promoter and the mouse metallothionein-I promoter appeared fivefold more active than the SV40 early promoter. In TOP cells the MoMLV LTR was found to be threefold more active than the Py early promoter. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|