Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System
| Autor: | Athanassios Fragoulis, Ivan Schlembach, Alan J. Slusarenko, Tim Caspers, Andreas Uebachs, Martin C.H. Gruhlke |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Phenethyl isothiocyanate antioxidant monomethyl fumarate phenylethyl isothiocyanate sulforaphane allicin lcsh:Technology electrophile lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine oxidative stress General Materials Science Luciferase Instrumentation Transcription factor lcsh:QH301-705.5 Fluid Flow and Transfer Processes YAP1 YKL071w lcsh:T Process Chemistry and Technology General Engineering luciferase allyl isothiocyanate Allyl isothiocyanate KEAP1 Yeast lcsh:QC1-999 Computer Science Applications Cell biology 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 kavalactones hepatocytes diethyl fumarate Yap1p lcsh:Engineering (General). Civil engineering (General) ddc:600 030217 neurology & neurosurgery phase II protective enzymes lcsh:Physics Sulforaphane |
| Zdroj: | Applied Sciences 10(10), 3520 (2020). doi:10.3390/app10103520 Applied Sciences, Vol 10, Iss 3520, p 3520 (2020) Applied Sciences Volume 10 Issue 10 |
| DOI: | 10.3390/app10103520 |
| Popis: | The oxidative stress response (OSR) in yeast is under the control of oxidation-sensitive cysteines in the Yap1p transcription factor, and fusion of the Yap1p-dependent OS-induced promoter of the YKL071w gene (OSI1) to a luciferase coding sequence makes a sensitive reporter for OS induced by electrophiles. In mammalian cells, the OSR induced by electrophiles is coordinated in a mechanistically similar way via oxidation-sensitive cysteines in the kelch-like ECH-associated protein 1 (Keap1)&ndash nuclear factor erythroid 2-related factor 2 / antioxidant response element ( Nrf2/ARE) system. Many electrophilic oxidants have already been independently shown to trigger both the Yap1 and Keap1 systems. Here, we investigated the responses of Yap1 and Keap1 reporters to sulforaphane (SFN), allyl isothiocyanate (AITC), phenylethyl isothiocyanate (PEITC), previously known to stimulate Keap1&ndash Nrf2/ARE but not known to activate Yap1, and as a positive control, allicin, previously reported to stimulate both Yap1 and Nrf2. We have compared the reciprocal responsiveness of the respective reporter systems and show that the yeast reporter system can have predictive value for electrophiles that stimulate the mammalian Keap1&ndash Nrf2/ARE system. |
| Databáze: | OpenAIRE |
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