RIN13-mediated disease resistance depends on the SNC1–EDS1/PAD4 signaling pathway in Arabidopsis
| Autor: | Jingjing He, Xiaoxiao Liu, Hui Han, Wen-Cheng Liu, Hui Liu, Zhiyong Gao, Siyuan Zhang, Yun-Kuan Liang, Zhangying Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Physiology Mutant Arabidopsis Dwarfism Plant Science Plant disease resistance 01 natural sciences Transcriptome 03 medical and health sciences Gene Expression Regulation Plant medicine Disease Resistance Plant Diseases Innate immune system biology Arabidopsis Proteins biology.organism_classification medicine.disease Cell biology DNA-Binding Proteins 030104 developmental biology Signal transduction Salicylic Acid Carboxylic Ester Hydrolases Signal Transduction 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany. 71:7393-7404 |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/eraa433 |
| Popis: | Plants have evolved an innate immune system to protect themselves from pathogen invasion with the help of intracellular nucleotide-binding leucine-rich repeat (NLR) receptors, though the mechanisms remain largely undefined. RIN13 (RPM1-interacting protein 13) was previously reported to enhance disease resistance, and suppress RPM1 (a CNL-type NLR)-mediated hypersensitive response in Arabidopsis via an as yet unknown mechanism. Here, we show that RIN13 is a nuclear-localized protein, and functions therein. Overexpression of RIN13 leads to autoimmunity with high accumulation of salicylic acid (SA), constitutive expression of pathogenesis-related genes, enhanced resistance to a virulent pathogen, and dwarfism. In addition, genetic and transcriptome analyses show that SA-dependent and SA-independent pathways are both required for RIN13-mediated disease resistance, with the EDS1/PAD4 complex as an integration point. RIN13-induced dwarfism was rescued completely by either the pad4-1 or the eds1-2 mutant but partially by snc1-r1, a mutant of the TNL gene SNC1, suggesting the involvement of EDS1/PAD4 and SNC1 in RIN13 functioning. Furthermore, transient expression assays indicated that RIN13 promotes the nuclear accumulation of PAD4. Collectively, our study uncovered a signaling pathway whereby SNC1 and EDS1/PAD4 act together to modulate RIN13-triggered plant defense responses. |
| Databáze: | OpenAIRE |
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