TrkB (Tropomyosin-Related Kinase B) Controls the Assembly and Maintenance of GABAergic Synapses in the Cerebellar Cortex
| Autor: | Sylvia Badurek, David R. Copenhagen, Cindy N Nguyen, Albert I. Chen, Barbara Ranscht, Louis F. Reichardt, Liliana Minichiello |
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| Rok vydání: | 2011 |
| Předmět: |
Scaffold protein
Neuroscience(all) Mice Transgenic Tropomyosin Tropomyosin receptor kinase B Neurotransmission Inhibitory postsynaptic potential Synaptic Transmission Cerebellar Cortex Mice Interneurons Cell Adhesion Animals Receptor trkB Cell adhesion gamma-Aminobutyric Acid Mice Knockout Gephyrin biology musculoskeletal neural and ocular physiology General Neuroscience Cell Differentiation Articles nervous system Cerebellar cortex Synapses embryonic structures biology.protein GABAergic Neuroscience |
| Zdroj: | Chen, A I, Nguyen, C N, Copenhagen, D R, Badurek, S, Minichiello, L, Ranscht, B & Reichardt, L F 2011, ' TrkB (tropomyosin-related kinase B) controls the assembly and maintenance of GABAergic synapses in the cerebellar cortex ', Journal of Neuroscience, vol. 31, no. 8, pp. 2769-80 . https://doi.org/10.1523/JNEUROSCI.4991-10.2011 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.4991-10.2011 |
| Popis: | Inhibitory interneurons play a critical role in coordinating the activity of neural circuits. To explore the mechanisms that direct the organization of inhibitory circuits, we analyzed the involvement of tropomyosin-related kinase B (TrkB) in the assembly and maintenance of GABAergic inhibitory synapses between Golgi and granule cells in the mouse cerebellar cortex. We show that TrkB acts directly within each cell-type to regulate synaptic differentiation. TrkB is required not only for assembly, but also maintenance of these synapses and acts, primarily, by regulating the localization of synaptic constituents. Postsynaptically, TrkB controls the localization of a scaffolding protein, gephyrin, but acts at a step subsequent to the localization of a cell adhesion molecule, Neuroligin-2. Importantly, TrkB is required for the localization of an Ig superfamily cell adhesion molecule, Contactin-1, in Golgi and granule cells and the absence of Contactin-1 also results in deficits in inhibitory synaptic development. Thus, our findings demonstrate that TrkB controls the assembly and maintenance of GABAergic synapses and suggest that TrkB functions, in part, through promoting synaptic adhesion. |
| Databáze: | OpenAIRE |
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