Inflammatory cytokine release of astrocytes in vitro is reduced by all-trans retinoic acid

Autor: Tommy Regen, Andrei Nemes, Uwe-Karsten Hanisch, Bernd Denecke, Philipp Imholz, Sonja Johann, Jörg Mey, Sabien van Neerven, Cordian Beyer
Rok vydání: 2010
Předmět:
Lipopolysaccharides
Chemokine
medicine.medical_treatment
Immunology
Anti-Inflammatory Agents
Retinoic acid
Antineoplastic Agents
Enzyme-Linked Immunosorbent Assay
Tretinoin
Retinoid X receptor
Dexamethasone
Mice
chemistry.chemical_compound
Glial Fibrillary Acidic Protein
medicine
Animals
Immunology and Allergy
Drug Interactions
RNA
Messenger
Annexin A2
Cells
Cultured
Oligonucleotide Array Sequence Analysis
Cerebral Cortex
Analysis of Variance
Mice
Inbred BALB C
L-Lactate Dehydrogenase
biology
Microglia
Gene Expression Profiling
Molecular biology
Retinoic acid receptor
Retinoid X Receptors
medicine.anatomical_structure
Cytokine
Animals
Newborn
Gene Expression Regulation
Neurology
chemistry
Astrocytes
biology.protein
Cytokines
Tumor necrosis factor alpha
Neurology (clinical)
Clodronic Acid
Astrocyte
Zdroj: Journal of Neuroimmunology. 229:169-179
ISSN: 0165-5728
DOI: 10.1016/j.jneuroim.2010.08.005
Popis: In the central nervous system inflammation is mediated by microglia and astrocytes. To investigate its regulation, murine astrocyte cultures were treated with bacterial lipopolysaccharides (LPS) and analyzed with Affymetrix gene array, qRT-PCR and ELISA. Cells responded to LPS with a strong upregulation of pro-inflammatory cytokines and chemokines. Treatment with the transcriptional activator retinoic acid (RA) suppressed mRNA expression and protein release of several important cytokines (IL-1β 4%, IL-6 21%, TNFα 30%, IL-12p40 42%, and IL-12p35/p40 27%; p
Databáze: OpenAIRE
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