A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy

Autor:	Jan R. R. Verlet, Diana L. Flindt, Aude Lietard, Caleb J. C. Jordan, Jacquie M. Robson, Martin A. Screen
Rok vydání:	2021
Předmět:	Materials science Absorption spectroscopy Fluorometer Analytical chemistry General Chemistry Spectroscopy Education
Zdroj:	Journal of Chemical Education, 2021, Vol.98(12), pp.3871-3877 [Peer Reviewed Journal]
ISSN:	1938-1328 0021-9584
DOI:	10.1021/acs.jchemed.1c00742
Popis:	A dual-function student-crafted instrument is described as part of a laboratory activity aimed to teach both the principles and practical aspects of absorption spectroscopy to secondary and introductory undergraduate students. Using minimal changes in an arrangement that is based on interlocking bricks and low-cost components, both a fluorometer and photometer have been constructed. The former demonstrates the principles of the Beer–Lambert law visually and quantitatively by acquiring the spatial light attenuation through a fluorescent sample. The latter then demonstrates its practical application in a visible-light spectrometer by measuring the absorption spectrum of an aqueous permanganate solution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98550bfe57642c664a326ec98e89d028 https://doi.org/10.1021/acs.jchemed.1c00742 Zobrazit plný text záznamu