Effect of fragaria vesca, hamamelis and tormentil on the initial bacterial colonization in situ
| Autor: | Amira Jung, Jasmin Kirsch, Belinda König, Matthias Hannig, Isabelle Kölling-Speer, Karl Speer, Kristin Hille, Christian Hannig |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
In situ Hamamelis Fragaria Bacterial Adhesion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bacterial colonization Animals Humans DAPI Food science Dental Pellicle General Dentistry Biofilm 030206 dentistry Cell Biology General Medicine 030104 developmental biology Otorhinolaryngology chemistry Polyphenol Biofilms Ultrastructure Cattle Test solution |
| Zdroj: | Archives of oral biology. 118 |
| ISSN: | 1879-1506 |
| Popis: | The presentin situ study aims to examine the influence of the polyphenolic tea drugs fragaria vesca, hamamelis and tormentil on the initial oral bioadhesion.Initial biofilm formation was performed on bovine enamel slabs which were carried intraorally by 12 subjects. After 1 min of intraoral pellicle formation, the subjects rinsed with fragaria vesca, tormentil (0.8 mg/8 mL) and hamamelis (0.2 mg/8 mL) for 10 min. Tap water served as negative control, 0.2 % CHX as positive control. The investigations took place on different days (wash-out: 2 days). Afterwards, fluorescence microscopy has been performed per test solution (n = 5) and per subject (n = 12) to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI, ConA and BacLight. Additionally, TEM was used to visualize the pellicle ultrastructure and expectorate samples. Statistical evaluation was carried out using the Kruskal-Wallis- (p 0.5), Mann-Whitney U test (p 0.5) and Bonferroni-Holm-correction (p 0.1).Rinsing with the polyphenolic tea extracts reduced significantly initial bacterial colonization (DAPI) compared to the negative control. There was no significant difference betweenfragaria vesca, hamamelis and tormentil. All solutions showed a reducing effect on the glucan formation. No significant difference was observed between fragaria vesca and CHX. Considerable alterations of the pellicle's ultrastructure manifested by an increase in thickness and electron density resulted from rinsing with the three polyphenolic aqueous extracts.Fragaria vesca, hamamelis and tormentil significantly reduce initial bioadhesion and glucan formation in situ and are therefore recommended as adjuvant antibacterial oral therapeutics. |
| Databáze: | OpenAIRE |
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