Kinetics of human immunodeficiency virus type 1 reverse transcription in blood mononuclear phagocytes are slowed by limitations of nucleotide precursors
Autor: | H Kalhor, William A. O'Brien, S H Mao, J A Zack, AM Namazi, I. S. Y. Chen |
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Rok vydání: | 1994 |
Předmět: |
AIDS Dementia Complex
Transcription Genetic Immunology Biology Virus Replication Polymerase Chain Reaction Microbiology Monocytes Virus Zidovudine Virology medicine Humans Macrophage Cells Cultured Nucleotides Cell growth Macrophages RNA-Directed DNA Polymerase T lymphocyte HIV Reverse Transcriptase Reverse transcriptase Viral replication Insect Science DNA Viral HIV-1 Research Article medicine.drug |
Zdroj: | Journal of Virology. 68:1258-1263 |
ISSN: | 1098-5514 0022-538X |
DOI: | 10.1128/jvi.68.2.1258-1263.1994 |
Popis: | Human immunodeficiency virus type 1 infection of mononuclear phagocytes has been implicated in disease manifestations, but postentry viral replication events in these cells have not been well characterized. Productive infection of activated T cells is associated with cell proliferation and accumulation of full-length viral DNA within 6 h. In infected, nondividing quiescent peripheral blood lymphocytes, reverse transcription is aborted prior to full-length viral DNA formation. For nondividing, cultured mononuclear phagocytes, we now report a third pattern of reverse transcription with relatively slow kinetics, in which full-length viral DNA did not accumulate until 36 to 48 h. The reverse transcription rate in mononuclear phagocytes could be accelerated by addition of exogenous nucleotide precursors, but still not to the rate seen in activated T cells. These results indicate that substrate limitations in mononuclear phagocytes slow but do not arrest human immunodeficiency virus type 1 reverse transcription. |
Databáze: | OpenAIRE |
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